If you mean lining up, for example, the Obtaining subgenres with the Obtaining variations, the answer is yes and no.
YES: like all of the quads, the subgenres obey TKAD, so they do broadly line up with the variations beneath them. Obtaining has Competitive (Approach), Security (Attitude), Benefit (Self Interest) and Rebalance (Morality) stories; Being has Persona (Desire) and Expectation (Ability) stories, etc. You absolutely can do it.
NO: But most of the stories don't line up that way. While some do, the majority tend to be elsewhere in the quad. Most of the Rebalance stories tend to be in the Self-Interest quad, for example. If all subgenres shared the same variation, it would become very 'samey' pretty quickly because every story would be dealing with the same issues.
My personal theory (and I haven't tried this) would be to tie the subgenres to the signposts. Whereas the Issues are variations on theme, the subgenres are variations on the story 'type' itself. I think the finesse you're looking for might be hidden in there, and would probably be less 'samey' -- because, for example, an Investigation story can be anything from a detective looking to find out what happened to his missing wife to a cowboy trying to find out which anthropomorphic horse drank his beer.
For example, if writing a dark comedy movie, you might have this:
Signpost 1 - Learning (Self-Discovery): After his wife abruptly divorces him, a high school teacher begins to see that he's wasted his life teaching kids that have gone on to be disappointments or criminals. His life's work is for nothing, and he is once again alone and miserable.
Signpost 2 - Doing (Endeavour): The teacher quits his job and turns to a life of adolescent joy: egging houses, shoplifting, spray painting. Anything that will give him a thrill.
Signpost 3 - Obtaining (Benefit): After getting a rush from shoplifting, the teacher turns to more and more intense 'lifts', breaking into the home of his former boss and stealing his rug. He's soon arrested when his boss checks in on him, and sees the rug firsthand.
Signpost 4 - Understanding (Appreciation): Unable to explain what happened, the teacher is placed in a correctional facility, where he is taught to understand basic concepts like 'why stealing is wrong' by a former 'disappointing' student.