The OS has 64 Elements spread throughout the players in a story.
The MC has 64 Elements used by the Main Character perspective only.
The IC has 64 Elements used by the Influence Character perspective only.
The RS has 64 Elements affecting the Relationship perspective only.
Each of the 64 Elements in any of these groups carries the same name as one in another group, but they are 256 different Elements. This is most visible when each Element is seen as an item within the context of the Variation under which it sits.
Whether there is a structure, order, or timing to the appearance of these Elements in a story is unknown to us. There very likely is, and there may be a proprietary algorithm to determine it. However, if there is such an algorithm, it has not been released to us and likely won't be. Thus, I can only answer from a general feel of stories. The OS characteristics seem to show up at least once per Signpost, while the other elements feel as though they weave throughout the story.
At the element level, though, the context becomes so blurred, buried, and creative that order virtually doesn't matter, even if there is a theoretic structure to it. Due to this minutia, trying to force a design generally produces a stilted story. Thus, considering I don't know your actual purpose in asking this question, I present these options.
If you're asking because you want to ensure that you create Complex Characters, then work with your muse to create characters that have unusual drives and purposes, and react in unexpected or strange ways.
If you are asking because you want to outline a story at this level, then check if you have outlined with these first: The Signposts, the Static Story Points, and the Plot Sequence Report. If so, then I would venture to guess that you have enough to start the actual writing at this point.
If you are asking in order to use the functions as Storytelling, then you've reached the limits of Dramatica. The theory covers structure, but gives very little for Storytelling. However, Storytelling is covered by virtually everyone else.
The truth is, the only decent amount of information we have is on the OS character functions. Any deeper dive into this portion of the theory is going to lead to a lot of guesswork. The information just isn't there.